تضخيم مواقعD18S51 و D8S1179 و FESلعينات الحمض النووي المعزولة من أوتار الجيتار المخزنة في درجة حرارة الغرفة
DOI:
https://doi.org/10.21123/bsj.2024.10206الكلمات المفتاحية:
D18S51، D8S1179، FES، STR Loci، أوتار الجيتار ، الجريمة.الملخص
الفعل الاجرامي هو سلوك غير قانوني وقد يكون جريمة جنائية ، ويستخدم المجرمون طرقا مختلفة لاخفاء الادلة في مسرح الجريمة لكن غالبا ما يجد خبراء الطب الشرعي اثار الحمض النووي على العناصر الموجودة في مسرح الجريمة, ومن هذه الادلة هو الجيتار. والجيتار من الالات الموسيقية التي يعزف عليها البشروالتي يمكن استخدامها للمقارنة . استخدمت الطرق في هذه الدراسة 18 عينة من أوتار الجيتار التي تم استخدامها لمدة خمس دقائق وحضنت في درجة حرارة الغرفة. تم بعد ذلك تقسيم العينات الـ 18 إلى ثلاث مجموعات، حيث تتكون كل مجموعة من ست عينات وتم تحضينها لمدة يوم وخمسة و10 أيام. تم بعد ذلك قياس الحمض النووي باستخدام مطياف الاشعة فوق البنفسجية للحمض النووي المستخلص بطريقة DNAzol . و كان معدل تقدير الحمض النووي في بعد الحضن لليوم الاول ( 152.57 ± 48.02 نانوغرام/ميكروليتر) واليوم 5 كانت ( 138.66 ± 47.66 نانوغرام/ميكروليتر)، واليوم 10 كانت ( كانت 87.09 ± 9.07 نانوغرام/ميكروليتر) . وتم الكشف عن كفاءة الحمض النووي المستخلص للمقارنة بتضخيم 3 مواقع هي D18S51 و FES و D8S1179 بتنقية تفاعل البلمرة المتسلسل، حيث ظهرت الحزم ذوات الاحجام ( 290-366) و((222 – 250 و( 203 – 224) زوج قاعدي, على التوالي. ونستنتج امكانية استخدام اوتار الجيتار كمصدر لعزل الحمض النووي بعد وقوع الجريمة بعدة ايام وبدرجة حرارة الغرفة.
Received 14/11/2023
Revised 07/07/2024
Accepted 09/07/2024
Published Online First 20/11/2024
المراجع
Daga SS, Ahmed U, Kumawat RK. Forensic Tools and Techniques of Absolute Human Identification: Physical and Molecular Approach. J Forensic Sci Crim Investig. 2021; 15(3): 1-6.
Turingan RS, Brown J, Kaplun L, et al. Identification of human remains using Rapid DNA analysis. Int J Legal Med. 2020; 134(3): 863-872. https://doi.org/10.1007/s00414-019-02186-y
Romeika JM. Recent Advances in Forensic DNA Analysis. J Forensic Res. 2013; s12(01). https://doi.org/10.4172/2157-7145.S12-001
Mahat N, Alwi A, Salleh F, Ishar S, Kamaluddin M, Rashid M. Applications of X-Chromosome Short Tandem Repeats for Human Identification: A Review. J Trop Life Sci. 2023; 13(1): 193-218. https://doi.org/10.11594/jtls.13.01.19
Hernández C. Mitochondrial DNA in Human Diversity and Health: From the Golden Age to the Omics Era. Genes (Basel). 2023; 14(8): 1534. https://doi.org/10.3390/genes14081534
Poma A, Cesare P, Bonfigli A, Volpe AR, Colafarina S, Vecchiotti G, et al. A qPCR-duplex assay for sex determination in ancient DNA. PLoS One. 2022; 17(6): e0269913. https://doi.org/10.1371/journal.pone.0269913
Yudianto A, Nuraini MI, Furqoni AH, Nzilibili SMM, Harjanto P. The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1, CSF1PO and TPOX. Indian J Forensic Med Toxicol. 2020; Jul 6; 12(Suppl 1): 8716. https://doi.org/10.37506/ijfmt.v14i3.10667
Furqoni AH, Megasari NLA, Yudianto A, Azizah F, Maulidiyanti ETS, Fahmi NF, et al. Amplification of vWA, FGA, and TH01 loci of DNA Samples Isolated From Ring Stored at Room Temperature. Malays J Med Health Sci. 2023; 19(5): 97-101.
Gosch A, Courts C. On DNA transfer: The lack and difficulty of systematic research and how to do it better. Forensic Sci Int Genet. 2019; 40: 24-36. https://doi.org/10.1016/j.fsigen.2019.01.012
Comte J, Baechler S, Gervaix J, Lock E, Milon MP, Delemont O, et al. Touch DNA collection – Performance of four different swabs. Forensic Sci Int Genet. 2019; 43: 102113. https://doi.org/10.1016/j.fsigen.2019.06.014
Li Y, Xie M, Wu J. DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation. Biomed Reports; 2014; 2(6): 804-808. doi:10.3892/br.2014.332
Puri P, Dubey M, Kumar N. DNA Profiling in Forensic Odontology. 1st ed. (Dash H, Shrivastava P, Lorente J, eds.). Springer Singapore; 2022. https://doi.org/10.1007/978-981-15-9364-2_63-1
Le TN, Muratovic D, Handt O, Henry J, Linacre A. DNA profiling from human bone cells in the absence of decalcification and DNA extraction. J Forensic Sci. 2022; 67(4): 1690-1696. doi:10.1111/1556-4029.15033
Udogadi NS, Abdullahi MK, Bukola AT, Imose OP, Esewi AD. Forensic DNA Profiling: Autosomal Short Tandem Repeat as a Prominent Marker in Crime Investigation. Malaysian J Med Sci. 2020; 27(4): 22-35. https://doi.org/10.21315/mjms2020.27.4.3
Dash HR, Arora M, Khatoon S. Evaluation of face masks as a valuable forensic DNA evidence in the post-COVID era. Int J Legal Med. 2023; 137(2): 311-317. https://doi.org/10.1007/s00414-023-02945-y
Tozzo P, Mazzobel E, Marcante B, Delicati A, Caenazzo L. Touch DNA Sampling Methods: Efficacy Evaluation and Systematic Review. Int J Mol Sci. 2022; 23(24): 15541. https://doi.org/10.3390/ijms232415541
Tang L. Primary Research on the Network Marketing of Musical Instrument Galleries. In: Proceedings of the 2012 2nd International Conference on Computer and Information Applications (ICCIA 2012). Atlantis Press; 2012. https://doi.org/10.2991/iccia.2012.206
Indonesian Supreme Court. PUTUSAN PN MATARAM 164/PID.B/2021/PN MTR. Published 2021.
Furqoni AH, Palupi R, Yudianto A, Sosiawan A, Tirthaningsih NW, Fore F, et al. Analysis of Sibling Pair Relationships of Balineses Indonesia, Using 12 STR Loci for Human Identification Process. J Int Dent Med Res. 2021; 14(2): 855-859. ISSN 1309-100X
Silva JATD. Room Temperature in Scientific Protocols and Experiments Should be Defined: a Reproducibility Issue. Biotechniques. 2021;70(6): 306-308. https://doi.org/10.2144/btn-2020-0131
Firmansyah NE, Thongseesuksai T, Boonmars T, Sungkar S, Laummaunwai P. Comparison of 3 DNA extraction methods for extracting DNA from an adult Culex quinquefasciatus (Diptera: Culicidae). J Insect Sci. 2023; 23(5): 15. https://doi.org/10.1093/jisesa/iead080
Minhas-Khan A, Ghafar-Zadeh M, Shaffaf T, Forouhi S, Scime A, Magierowski S, et al. UV-Vis Spectrophotometric Analysis of DNA Retrieval for DNA Storage Applications. Actuators. 2021; 10(10): 246. https://doi.org/10.3390/act10100246
García-Alegría AM, Anduro-Corona I, Pérez-Martínez CJ, Guadalupe Corella-Madueño MA, Rascón-Durán ML, Astiazaran-Garcia H. Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA. Int J Anal Chem. 2020; 1-9. https://doi.org/10.1155/2020/8896738
Jiang Y, Zheng L, Lin L, Lin S, Xu K, Deng S, et al. Modification in Silver Staining Procedure for Enhanced Protein Staining. Teekaraman Y, ed. Biomed Res Int. 2022; 1-9. https://doi.org/10.1155/2022/6243971
Hanssen EN, Lyle R, Egeland T, Gill P. Degradation in forensic trace DNA samples explored by massively parallel sequencing. Forensic Sci Int Genet. 2017; 27: 160-166. https://doi.org/10.1016/j.fsigen.2017.01.002
Bhoyar L, Mehar P, Chavali K. An overview of DNA degradation and its implications in forensic caseworks. Egypt J Forensic Sci. 2024; 14(1): 15. https://doi.org/10.1186/s41935-024-00389-y
Naef T, Besnard A, Lehnen L, Petit EJ, van Schaik J, Puechmaille SJ. How to quantify factors degrading DNA in the environment and predict degradation for effective sampling design. Environ DNA. 2023; 5(3): 403-416. https://doi.org/10.1002/edn3.414
Zhu G, Guo YA, Ho D, Poon P, Poh ZW, Wong PM, et al. Tissue-specific cell-free DNA degradation quantifies circulating tumor DNA burden. Nat Commun. 2021; 12(1): 2229. https://doi.org/10.1038/s41467-021-22463-y
Abdel Hady RH, Thabet HZ, Ebrahem NE, Yassa HA. Thermal Effects on DNA Degradation in Blood and Seminal Stains: Forensic View. Acad Forensic Pathol. 2021; 11(1): 7-23. https://doi.org/10.1177/1925362121998547
Samehsalari S, Chandrasekar A. Muslims from South India. J Genet Resour. 2021; 7(2): 220-226. https://doi.org/10.22080/jgr.2021.20887.1239
Yudianto A, Erliyah T, Furqoni AH, Nuraini I, Huda QA. Effect of Storage Time on DNA Content and Purity in Lip Print. J Biosains Pascasarj. 2023; 25(1): 43-48. https://doi.org/10.20473/jbp.v25i1.2023.43-48
Divya K, GK M, Umema A, SS D. Environmental Factors Affecting the Concentration of DNA in Blood and Saliva Stains: A Review. J Forensic Sci Res. 2024; 8(1): 009-015. https://doi.org/10.29328/journal.jfsr.1001057
Khoury M El. The Effects of Heat and Explosions on Forensic DNA Analyses by The Effects of Heat and Explosions on Forensic DNA Analyses (PhD Dissertation). University of Leicester; 2020.
Aflanie I, Prastowo W, Panghiyangani R, Yudianto A, Koesbardiati T. Genetic variation analysis and kinship relationship between Dayak Ngaju tribe and Dayak Bukit tribe through examination of core DNA of Bukit CODIS STR Locus (combine DNA index system) 13 for the purpose of Forensic Identification. Indian J Forensic Med Toxicol; 2020; 14(3): 1094-1097. https://doi.org/10.37506/ijfmt.v14i3.10519
Manela C, Susanti R, Tjong DH, Yudianto A. Genetic Analysis 21 Short Tandem Repeats (STR) Locus in Minangkabau Population, West Sumatera, Indonesia. African J Infect Dis. 2022; 16(2): 35-41. https://doi.org/10.21010/Ajid.v16i2.4
Wyner N, Barash M, McNevin D. Forensic Autosomal Short Tandem Repeats and Their Potential Association With Phenotype. Front Genet. 2020;11: 884. https://doi.org/10.3389/fgene.2020.00884
Arimurti ARR, Yudianto A, Astuti SP. Identifikasi DNA dari Swab Earphone dengan Teknik STR (Short Tandem Repeat) untuk Kepentingan Forensik. J Biosains Pascasarj. 2015; 17(1): 26-32. https://doi.org/10.20473/jbp.v17i1.2015.26-32
Sosiawan A, Yudianto A, Furqoni AH, Nzilibili SMM, Nuraini I. Full-sibling allelic frequency and sharing among Madurese: STR technique by 12 locus and the sex-typing amelogenin gene. Egypt J Forensic Sci. 2019; 9(1): 38. https://doi.org/10.1186/s41935-019-0143-5
التنزيلات
إصدار
القسم
الرخصة
الحقوق الفكرية (c) 2024 Abdul Hadi Furqoni, Anindita Riesti Retno Arimurti, Meri Meri, Ni Luh Ayu Megasari, Indah Nuraini Masjkur, Qurrota A’yunil Huda, Intan Sari Nuraini, Luluk Hermawati
هذا العمل مرخص بموجب Creative Commons Attribution 4.0 International License.
كيفية الاقتباس
